| 存储条件 |
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| Product Name | CK5 [6D-4D] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 58 kDa cytokeratin antibody CK-5 antibody CK5 antibody Cytokeratin-5 antibody Cytokeratin5 antibody DDD antibody DDD1 antibody EBS2 antibody epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody K2C5_HUMAN antibody K5 antibody keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody Keratin 5 antibody Keratin antibody keratin complex 2, basic, gene 5 antibody keratin, type II cytoskeletal 5 antibody Keratin-5 antibody Keratin5 antibody KRT 5 antibody Krt5 antibody KRT5A antibody type II cytoskeletal 5 antibody Type-II keratin Kb5 antibody |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | 58 kDa cytokeratin antibody CK-5 antibody CK5 antibody Cytokeratin-5 antibody Cytokeratin5 antibody DDD antibody DDD1 antibody EBS2 antibody epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody K2C5_HUMAN antibody K5 antibody keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types) antibody Keratin 5 antibody Keratin antibody keratin complex 2 basic gene 5 antibody keratin type II cytoskeletal 5 antibody Keratin-5 antibody Keratin5 antibody KRT 5 antibody Krt5 antibody KRT5A antibody type II cytoskeletal 5 antibody Type-II keratin Kb5 antibody |
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| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of CK5 on different cells lysates using anti-CK5 antibody at 1/1,000 dilution. Positive control： Line1: A431 Line2: 3T3-L1 Line3: COS7 Line4: MCF-7 Line5: SK-Br-3 Line6: Hela Line7: Lncap Line8: HepG2
Application
Fig2: Western blot analysis of CK5 on human CK5 recombinant protein using anti-CK5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CK5 on HEK293 (1) and CK5-hIgGFc transfected HEK293 (2) cell lysate using anti-CK5 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining CK5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- CK5 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with CK5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | A431, 3T3-L1, COS7, HepG2, Hela, MCF-7, SK-Br-3, Lncap, human CK5 recombinant protein, human ovarian cancer tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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