| 存储条件 |
|---|
| Product Name | Caspase-6 [SC56-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptotic protease Mch-2 antibody Apoptotic protease MCH2 antibody CASP-6 antibody CASP6 antibody CASP6_HUMAN antibody Caspase 6 antibody Caspase 6 apoptosis related cysteine protease antibody Caspase 6, apoptosis related cysteine peptidase antibody Caspase-6 subunit p11 antibody Mch2 antibody OTTHUMP00000162742 antibody OTTHUMP00000162743 antibody |
| Product description | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Apoptotic protease Mch-2 antibody Apoptotic protease MCH2 antibody CASP-6 antibody CASP6 antibody CASP6_HUMAN antibody Caspase 6 antibody Caspase 6 apoptosis related cysteine protease antibody Caspase 6 apoptosis related cysteine peptidase antibody Caspase-6 subunit p11 antibody Mch2 antibody OTTHUMP00000162742 antibody OTTHUMP00000162743 antibody |
|---|---|
| Molecular Weight(MW) | 11/33 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Caspase-6 on different lysates using anti-Caspase-6 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse colon Lane 2: MCF-7
Application
Fig2: ICC staining Caspase-6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin.| Positive Control | SW480, MCF-7, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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