| 存储条件 |
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| Product Name | WNT3A [EG-64] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Wnt 3a Precursor antibody Protein Wnt-3a antibody Wingless type MMTV integration site family member 3A antibody Wnt 3a antibody wnt3a antibody WNT3A protein antibody WNT3A_HUMAN antibody |
| Product description | The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. The Wnt family has two functional classes according to their biological activities; Wnts that signal through a Wnt-1/wingless pathway by stabilizing cytoplasmic beta-catenin, and Wnts that stimulate intracellular Ca2+ release and activate two kinases, CamKII and PKC, in a G protein-dependent manner. Wnt-3a is an intercellular signaling molecule that mediates cytoskeletal reorganization and regulates hippocampal development. Human Wnt-3a is 96% homologous to mouse Wnt-3a protein and 84% homologous to human Wnt-3 protein. The human Wnt-3a gene clusters with the Wnt-14 gene at chromosome 1q42. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Protein Wnt 3a Precursor antibody Protein Wnt-3a antibody Wingless type MMTV integration site family member 3A antibody Wnt 3a antibody wnt3a antibody WNT3A protein antibody WNT3A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| SwissProt ID | P56704 |
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Application
Fig1: Western blot analysis of WNT3A on human WNT3A recombinant protein using anti-WNT3A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of WNT3A on HEK293 (1) and WNT3A-hIgGFc transfected HEK293 (2) cell lysate using anti-WNT3A antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of WNT3A on different cells lysate using anti-WNT3A antibody at 1/1,000 dilution. Positive control： Line1: A549 Line2: HepG2 Line3: A431
Application
Fig4: ICC staining WNT3A (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with WNT3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human WNT3A recombinant protein, WNT3A-hIgGFc transfected HEK293 cell lysate,A549, HepG2, A431, Hela, human rectum cancer tissue, human endometrial cancer tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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