| 存储条件 |
|---|
| Product Name | Pan-Cadherin [ST54-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin antibody CDH3 antibody CDHP antibody 7B4 antigen antibody Cadherin-1 antibody Cadherin-2 antibody Cadherin-3 antibody Cadherin-4 antibody Cadherin-5 antibody CAM 120/80 antibody CD144 antibody CD324 antibody CD325 antibody Cdh4 antibody CDH5 antibody CDHE antibody CDHN antibody E-cadherin antibody Epithelial cadherin antibody N-cadherin antibody NCAD antibody Neural cadherin antibody P-cadherin antibody Placental cadherin antibody UVO antibody Uvomorulin antibody Vascular endothelial cadherin antibody |
| Product description | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH;2; terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin, R-cadherin, B-cadherin, E/P cadherin and cadherin-5. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cadherin antibody CDH3 antibody CDHP antibody 7B4 antigen antibody Cadherin-1 antibody Cadherin-2 antibody Cadherin-3 antibody Cadherin-4 antibody Cadherin-5 antibody CAM 120/80 antibody CD144 antibody CD324 antibody CD325 antibody Cdh4 antibody CDH5 antibody CDHE antibody CDHN antibody E-cadherin antibody Epithelial cadherin antibody N-cadherin antibody NCAD antibody Neural cadherin antibody P-cadherin antibody Placental cadherin antibody UVO antibody Uvomorulin antibody Vascular endothelial cadherin antibody |
|---|---|
| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | Cell membrane, Cell junction, Endosome, Golgi apparatus. |
Application
Fig1: Western blot analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart
Application
Fig2: ICC staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, mouse liver tissue, mouse kidney tissue, rat heart tissue, human heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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