CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2. CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli. CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes. LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases.
Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Jurkat cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human CD68 recombinant protein, CD68-hIgGFc transfected HEK293 cell lysate, Jurkat.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
