| 存储条件 |
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| Product Name | TPA [AH81-00] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alteplase antibody DKFZp686I03148 antibody Plasminogen activator tissue antibody Plasminogen activator tissue type antibody PLAT antibody Reteplase antibody t PA antibody T Plasminogen Activator antibody t-PA antibody T-plasminogen activator antibody Tissue plasminogen activator (t PA) antibody Tissue type plasminogen activator antibody Tissue-type plasminogen activator chain B antibody tPA antibody TPA_HUMAN antibody TPA1 antibody |
| Product description | uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), which are serine proteases and members of the trypsin family, are essential to the intrinsic coagulation system. tPA is primarily involved in fibrinolysis, whereas uPA principally mediates cell migration and tissue remodeling processes. uPA and tPA are responsible for cleaving plasminogen, a large serum β-globulin that is deposited on the Fibrin strands within a thrombus. uPA and tPA preferentially target plasminogen at the Arg-Val bond to produce plasmin (also designated fibrinolysin), which is a trypsin-like enzyme that acts on Arg-Lys bonds in Fibrin and Fibrinogen and contributes to the systematic activation of the coagulation cascade. uPA and tPA each consist of two chains that are designated A and B. The A chain of uPA can be cleaved, resulting in low and high molecular mass forms. uPA and tPA are regulated by the serpin family members PAI-1 and PAI-2, which are serine proteinase inhibitors that complex with uPA, tPA and other targeted proteinases and then slowly disassociate to produce cleaved species that fold into stable inactive conformations. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBICCIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1,5,000-1:10,000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Alteplase antibody DKFZp686I03148 antibody Plasminogen activator tissue antibody Plasminogen activator tissue type antibody PLAT antibody Reteplase antibody t PA antibody T Plasminogen Activator antibody t-PA antibody T-plasminogen activator antibody Tissue plasminogen activator (t PA) antibody Tissue type plasminogen activator antibody Tissue-type plasminogen activator chain B antibody tPA antibody TPA_HUMAN antibody TPA1 antibody |
|---|---|
| Molecular Weight(MW) | 62/30 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: ICC staining TPA in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining TPA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TPA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-TPA antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TPA antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TPA antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-TPA Tubulin antibody. Counter stained with hematoxylin.| Positive Control | A549, HepG2, N2A, human liver, mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1,5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.22% Sodium Azide. |
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