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| Product Name | Phospho-MEK1(S218/S222) [ST0490] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity mitogen activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP2K1 antibody MAPK/ERK kinase 1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody Mek1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody MKK 1 antibody MKK1 antibody MP2K1_HUMAN antibody PRKMK1 antibody Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody Protein kinase mitogen activated, kinase 1 antibody hide |
| Product description | Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of human MEK1. |
| Modification | p-S218/S222 |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Dual specificity mitogen activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP2K1 antibody MAPK/ERK kinase 1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody Mek1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody MKK 1 antibody MKK1 antibody MP2K1_HUMAN antibody PRKMK1 antibody Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody Protein kinase mitogen activated kinase 1 antibody hide |
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| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane. |
| SwissProt ID | Q02750 |
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Application
Fig1: Western blot analysis of Phospho-MEK1(S218/S222) on different lysates using anti-Phospho-MEK1(S218/S222) antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Hela Lane 3: 293T
Application
Fig2: ICC staining Phospho-MEK1(S218/S222) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-MEK1(S218/S222) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Phospho-MEK1(S218/S222) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, A431, Hela, HepG2, human spleen tissue, human tonsil tissue, human breast tissue, human liver cancer tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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