| 存储条件 |
|---|
| Product Name | IL7 [AH34-12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL 7 antibody IL-7 antibody Il7 antibody IL7_HUMAN antibody Interleukin 7 antibody Interleukin-7 antibody LP-1 antibody Lymphopoietin 1 antibody |
| Product description | Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor γ chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2), originally called 4PS. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBICCIHC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 | |
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | IL 7 antibody IL-7 antibody Il7 antibody IL7_HUMAN antibody Interleukin 7 antibody Interleukin-7 antibody LP-1 antibody Lymphopoietin 1 antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of IL7 on human thymus cell lysates using anti- IL7 antibody at 1/500 dilution.
Application
Fig2: ICC staining IL7 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IL7 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IL7 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining IL7 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL7 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.| Positive Control | 293T, Hela, HepG2, MCF-7, human thymus cell lysates, human colon cancer tissue, rat spleen tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.17% Sodium Azide. |
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