| 存储条件 |
|---|
| Product Name | Histone H2A.Z [ST46-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2A histone family member Z H2A.z H2A/z H2afz H2AZ H2AZ_HUMAN Histone H2A.Z MGC117173 |
| Product description | Histone H2A.Z/H2A.F/Z (H2A/z) is a 128 amino acid protein encoded by the human gene H2AFZ. Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA sequentially in a left-handed super-helical turn to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, creating two nearly symmetrical halves by tertiary structure. H2A.Z/H2A.F/Z is a variant Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in tran-scription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of posttranslational modifications of histones, also called histone code, and nucleosome remodeling. H2A.Z/H2A.F/Z may be involved in the formation of constitutive heterochromatin and may be required for chromosome segregation during cell division. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICC/IF |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | H2A histone family member Z H2A.z H2A/z H2afz H2AZ H2AZ_HUMAN Histone H2A.Z MGC117173 |
|---|---|
| Molecular Weight(MW) | 13 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: ICC staining Histone H2A.Z in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PARK7 antibody. Counter stained with hematoxylin.| Positive Control | Hela, human spleen tissue, mouse colon tissue, human lung cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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