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| Product Name | YB1 [ST0432] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | BP 8 antibody CBF-A antibody CCAAT binding transcription factor I subunit A antibody CCAAT-binding transcription factor I subunit A antibody CSDA2 antibody CSDB antibody DBPB antibody DNA binding protein B antibody DNA-binding protein B antibody EFI-A antibody Enhancer factor I subunit A antibody MDR NF1 antibody MGC104858 antibody MGC110976 antibody MGC117250 antibody NSEP 1 antibody NSEP1 antibody Nuclease sensitive element binding protein 1 antibody Nuclease-sensitive element-binding protein 1 antibody p50 antibody Q15905 antibody Y-box binding protein 1 antibody Y-box transcription factor antibody Y-box-binding protein 1 antibody YB 1 antibody YB-1 antibody YBOX1_HUMAN antibody YBX 1 antibody ybx1 antibody |
| Product description | Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BP 8 antibody CBF-A antibody CCAAT binding transcription factor I subunit A antibody CCAAT-binding transcription factor I subunit A antibody CSDA2 antibody CSDB antibody DBPB antibody DNA binding protein B antibody DNA-binding protein B antibody EFI-A antibody Enhancer factor I subunit A antibody MDR NF1 antibody MGC104858 antibody MGC110976 antibody MGC117250 antibody NSEP 1 antibody NSEP1 antibody Nuclease sensitive element binding protein 1 antibody Nuclease-sensitive element-binding protein 1 antibody p50 antibody Q15905 antibody Y-box binding protein 1 antibody Y-box transcription factor antibody Y-box-binding protein 1 antibody YB 1 antibody YB-1 antibody YBOX1_HUMAN antibody YBX 1 antibody ybx1 antibody |
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| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cytoplasmic granule, Secreted. |
Application
Fig1: Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat
Application
Fig2: ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, HepG2, human breast carcinoma tissue, human kidney tissue, mouse colon tissue, mouse stomach tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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