| 存储条件 |
|---|
| Product Name | beta 2 Microglobulin [SU35-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B2M antibody B2MG_HUMAN antibody Beta 2 microglobin antibody Beta 2 microglobulin antibody Beta 2 microglobulin precursor antibody Beta chain of mhc class 1 proteins antibody Beta chain of MHC class I molecules antibody Beta-2-microglobulin form pI 5.3 antibody CDABP0092 antibody Hdcma22p antibody |
| Product description | Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an a heavy chain that contains three subdomains (α1, α2, α3), and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the a3 subdomain of the a heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the a heavy chain form the peptide antigen-binding cleft. Mice that lack β-2-Microglobulin protein show a normal distribution of T cells, yet have no mature CD4-8+ T cells and are defective in CD4-8+ T cell-mediated cytotoxicity. Interferon-γ can stimulate production of β-2-Microglobulin transcripts. The human β-2-Microglobulin gene maps to chromosome 15q21-q22.2 and encodes a 119 amino acid protein. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | B2M antibody B2MG_HUMAN antibody Beta 2 microglobin antibody Beta 2 microglobulin antibody Beta 2 microglobulin precursor antibody Beta chain of mhc class 1 proteins antibody Beta chain of MHC class I molecules antibody Beta-2-microglobulin form pI 5.3 antibody CDABP0092 antibody Hdcma22p antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: U937
Application
Fig2: ICC staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining beta 2 Microglobulin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A431, Raji, Hela, U937. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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