| 存储条件 |
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| Product Name | ERp72 [10-F2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium binding protein intestinal related antibody Endoplasmic reticulum resident protein 70 antibody Endoplasmic reticulum resident protein 72 antibody EPR70 antibody EPR72 antibody ER protein 70 antibody ER protein 72 antibody ERP 70 antibody ERp 72 antibody ERp-72 antibody ERp70 antibody ERp72 antibody PDIA 4 antibody Pdia4 antibody PDIA4_HUMAN antibody PDIR antibody Protein disulfide isomerase A4 antibody Protein disulfide isomerase associated 4 antibody Protein disulfide isomerase family A member 4 antibody protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody Protein disulfide isomerase related protein antibody Protein disulfide-isomerase A4 antibody Protein ERp 72 antibody Protein ERp72 antibody |
| Product description | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. |
| Immunogen | Synthetic peptide within C-terminal human ERp72. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:1,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Calcium binding protein intestinal related antibody Endoplasmic reticulum resident protein 70 antibody Endoplasmic reticulum resident protein 72 antibody EPR70 antibody EPR72 antibody ER protein 70 antibody ER protein 72 antibody ERP 70 antibody ERp 72 antibody ERp-72 antibody ERp70 antibody ERp72 antibody PDIA 4 antibody Pdia4 antibody PDIA4_HUMAN antibody PDIR antibody Protein disulfide isomerase A4 antibody Protein disulfide isomerase associated 4 antibody Protein disulfide isomerase family A member 4 antibody protein disulfide isomerase related protein (calcium-binding protein intestinal-related) antibody Protein disulfide isomerase related protein antibody Protein disulfide-isomerase A4 antibody Protein ERp 72 antibody Protein ERp72 antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
Application
Fig2: ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | A431, HepG2, SiHa, SKOV-3, human lung cancer tissue, human liver tissue, rat testis tissue, human placenta tissue, mouse brain tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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