| 存储条件 |
|---|
| Product Name | Cleaved PARP [SU0314] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP-ribosyltransferase diphtheria toxin-like 1 antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody APOPAIN antibody ARTD1 antibody NAD(+) ADP-ribosyltransferase 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly [ADP-ribose] polymerase 1 antibody Poly ADP ribose polymerase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody SCA1 antibody |
| Product description | Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIPFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ADP-ribosyltransferase diphtheria toxin-like 1 antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody APOPAIN antibody ARTD1 antibody NAD(+) ADP-ribosyltransferase 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly [ADP-ribose] polymerase 1 antibody Poly ADP ribose polymerase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody SCA1 antibody |
|---|---|
| Molecular Weight(MW) | 89 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P09874 |
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Application
Fig1: Western blot analysis of Cleaved PARP on diferent lysates using anti-Cleaved PARP antibody at 1/1,000 dilution.Positive control：Lane1: Jurkat Lane2: A549
Application
Fig2: Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Jurkat |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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