| 存储条件 |
|---|
| Product Name | AMACR/P504S [6F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2 arylpropionyl CoA epimerase antibody 2 methylacyl CoA racemase antibody 2-methylacyl-CoA racemase antibody Alpha methylacyl CoA racemase antibody Alpha methylacyl Coenzyme A racemase antibody Alpha methylacyl-CoA racemase deficiency, included antibody Alpha-methylacyl-CoA racemase antibody Amacr antibody AMACR deficiency, included antibody AMACR_HUMAN antibody CBAS4 antibody Da1-8 antibody EC 5.1.99.4 antibody Macr1 antibody Methylacyl CoA racemase alpha antibody RACE antibody RM antibody |
| Product description | AMACR, is an enzyme that responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. Both decreased and increased levels of the enzyme in humans are linked with diseases. Reduction of the protein level or activity results in the accumulation of (2R)-methyl fatty acids such as bile acids which causes neurological symptoms. Increased levels of AMACR protein concentration and activity are associated with prostate cancer, and the enzyme is used widely as a biomarker (known in cancer literature as P504S) in biopsy tissues. Increased levels of AMACR are also associated with some breast, colon, and other cancers, but it is unclear exactly what the role of AMACR is in these cancers. This AMACR antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods. |
| Immunogen | Recombinant full length protein of human AMACR. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 2 arylpropionyl CoA epimerase antibody 2 methylacyl CoA racemase antibody 2-methylacyl-CoA racemase antibody Alpha methylacyl CoA racemase antibody Alpha methylacyl Coenzyme A racemase antibody Alpha methylacyl-CoA racemase deficiency included antibody Alpha-methylacyl-CoA racemase antibody Amacr antibody AMACR deficiency included antibody AMACR_HUMAN antibody CBAS4 antibody Da1-8 antibody EC 5.1.99.4 antibody Macr1 antibody Methylacyl CoA racemase alpha antibody RACE antibody RM antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Mitochondrion. Peroxisome. |
| SwissProt ID | Q9UHK6 |
|---|
Application
Fig1: Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with AMACR/P504S antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | Siha cell and human liver tissue lysates, Human liver tissue, human colon cancer tissue, human prostate cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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