| 存储条件 |
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| Product Name | IL-31 [8-C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL 31 antibody IL-31 antibody IL31 antibody IL31_HUMAN antibody Interleukin 31 antibody Interleukin-31 antibody |
| Product description | IL-31 is a T cell cytokine that is preferentially produced by T helper type 2 cells. IL-31 signals through a heterodimeric receptor composed of the IL-31 receptor (IL-31R) and the oncostatin M receptor (OSM). This receptor complex recruits JAK1, JAK2, Stat1, Stat3 and Stat5 signaling pathways, as well as the PI3 kinase/AKT cascade. SHP-2 and Shc adapter molecules are also recruited and contribute to an increased activation of the MAP kinase pathway in response to IL-31. Overexpression of IL-31 in mice results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Comparisons between skin from patients with AD and healthy skin showed IL-31R expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL-31 mRNA. IL-31 may participate in the cause of itch sensation and promote scratching behavior in NC/Nga mice with atopic dermatitis, and may represent a novel target for antipruritic drug development. |
| Immunogen | Recombinant full length protein corresponding to human IL18. |
| Clonality | Monoclonal |
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| Isotype | IgG3 |
| Host Species | Mouse |
| Tested Applications | WBIHCICC |
| WB:1:500 ICC:1:100 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | IL 31 antibody IL-31 antibody IL31 antibody IL31_HUMAN antibody Interleukin 31 antibody Interleukin-31 antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q6EBC2 |
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Application
Fig1: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.
Application
Fig2: ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-31 antibody. Counter stained with hematoxylin.| Positive Control | Recombinant protein lysate, A431, LOVO, MCF-7, human tonsil tissue, human spleen tissue. |
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| Application Notes | WB:1:500 ICC:1:100 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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