| 存储条件 |
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| Product Name | alpha smooth muscle Actin [SY02-64] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin, aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody |
| Product description | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: NIH/3T3
Application
Fig2: ICC staining alpha smooth muscle Actin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining alpha smooth muscle Actin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining alpha smooth muscle Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining alpha smooth muscle Actin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Jurkat cells with alpha smooth muscle Actin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A431, NIH/3T3, Hela, HepG2, RH-35, A549, human gastric carcinoma tissue, human tonsil tissue, human liver tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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