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| Product Name | UGP2 Mouse Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | UGP2 (UDP-glucose pyrophosphorylase 2), also known as UDPG, UGPP2, UDPGP2 or pHC379, is an evolutionarily conserved protein belonging to the UDPGP type 1 family of proteins. Localizing to the cytoplasm, UGP2 forms homooligomers and is believed to function as a glucosyl donor in cellular metabolic pathways. More specifically, UGP2 catalyzes the transfer of a glucose moiety from glucose-1-phosphate to UTP, producing a diphosphate and UDP-glucose. UDP-glucose is an essential precursor for the synthesis of polysaccharides; in liver and muscle, UDP-glucose is a precursor of glycogen, in liver UDP-glucose is also a precursor of UDP-glucuronate, and in lactating mammary gland UDP-glucose is converted to UDP-galactose and then to lactose. |
| Immunogen | Synthetic peptide within Human UGP2 aa 61-110 / 508. |
| Clonality | monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:50-1:400 ICC/IF:1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | pHC379 antibody UDP glucose diphosphorylase antibody UDP glucose pyrophosphorylase 1 antibody UDP glucose pyrophosphorylase 2 antibody UDP glucose pyrophosphorylase antibody UDP-glucose pyrophosphorylase antibody UDPG antibody UDPGP 2 antibody UDPGP antibody UDPGP2 antibody UGP 1 antibody UGP 2 antibody UGP1 antibody Ugp2 antibody UGPA_HUMAN antibody UGPase 2 antibody UGPase antibody UGPP 1 antibody UGPP 2 antibody UGPP1 antibody UGPP2 antibody Uridyl diphosphate glucose pyrophosphorylase 1 antibody Uridyl diphosphate glucose pyrophosphorylase 2 antibody UTP glucose 1 phosphate uridyltransferase antibody UTP glucose 1 phosphate uridylyltransferase 2 antibody UTP glucose 1 phosphate uridylyltransferase antibody UTPglucose-1-phosphate uridylyltransferase antibody |
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| Molecular Weight(MW) | 57 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of UGP2 on human liver tissue lysates with Mouse anti-UGP2 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-UGP2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-UGP2 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-UGP2 antibody. Counter stained with hematoxylin.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling UGP2 with Mouse anti-UGP2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-UGP2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:50-1:400 ICC/IF:1:100 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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