| 存储条件 |
|---|
| Product Name | Histone H3 [A11-D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:5,000-1:10,000 ICC:1:200-1:500 | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Molecular Weight(MW) | 17 kDa |
|---|---|
| Cellular Localization | Nucleus |
| SwissProt ID | P68431 |
|---|
Application
Fig1: Western blot analysis of Histone H3 on different cell lysates using anti-Histone H3 antibody at 1/2,000 dilution.Positive control:Lane 1: HelaLane 2: PC-12Lane 3: F9
Application
Fig2：Immunofluorescent staining of Hela cells using anti-Histone H3 Mouse mAb.
Application
Fig3：Immunofluorescent staining of F9 cells using anti-Histone H3 Mouse mAb.
Application
Fig4: Western blot analysis of Histone H3 on hybrid fish (crucian-carp) brain tissue lysate using anti-Histone H3 antibody at 1/500 dilution.| Positive Control | Hela, PC-12, F9, hybrid fish (crucian-carp) brain tissue lysate. |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 ICC:1:200-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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