| 存储条件 |
|---|
| Product Name | GFP [SP069-0] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GFP antibody Green fluorescent protein antibody yfp antibody |
| Product description | The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500~1:2000 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | GFP antibody Green fluorescent protein antibody yfp antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
Application
Fig1: Western blot analysis of GFP on K562 cells transfected with GFP using anti-GFP antibody at 1/10,000 dilution.| Positive Control | K562 cells transfected with GFP. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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