| 存储条件 |
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| Product Name | PCSK9 [2F1] |
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| Antibody Type | Primary Antibodies |
| Product description | Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC-1, is a 692 amino acid protein that belongs to the peptidase S8 family and contains one peptidase S8 domain. Important in the regulation of plasma cholesterol homeostasis, PCSK9 binds to low-density lipid receptor family members LDLR, very low-density lipid receptor (VLDLR) and apolipoprotein receptor 2 (ApoER2) and promotes their degradation in intracellular acidic compartments. PCSK9 also plays a role in neuronal differentiation and apoptosis. PCSK9 is expressed in Shwann cells, neuro-epithelioma, colon carcinoma, and hepatic and pancreatic cell lines. PCSK9 levels in the brain are highest in the cerebellum during perinatal development, with ischemia causing increased levels in the adult brain. Defects in the gene encoding this protein causes the autosomal dominant disorder familial hypercholesterolemia 3 (HCHOLA3). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Convertase subtilisin/kexin type 9 preproprotein antibody FH3 antibody HCHOLA3 antibody Hypercholesterolemia autosomal dominant 3 antibody LDLCQ1 antibody NARC 1 antibody NARC-1 antibody NARC1 antibody Neural apoptosis regulated convertase 1 antibody Neural apoptosis-regulated convertase 1 antibody PC 9 antibody PC9 antibody PCSK 9 antibody PCSK9 antibody PCSK9_HUMAN antibody Proprotein convertase 9 antibody Proprotein convertase PC9 antibody Proprotein convertase subtilisin/kexin type 9 antibody PSEC0052 antibody Subtilisin/kexin like protease PC9 antibody Subtilisin/kexin-like protease PC9 antibody |
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| Molecular Weight(MW) | 60 kDa |
Application Fig1: Western blot analysis of PCSK9 on PANC-1 using anti-PCSK9 antibody at 1/100 dilution.
Application Fig2: Western blot analysis of PCSK9 on H22 (1) and SW1990 (2) using anti-PCSK9 antibody at 1/500 dilution.
Application Fig3: ICC staining PCSK9 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining PCSK9 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human colon caner tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
Application Fig9:Flow cytometric analysis of Hela cells with PCSK9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
| Positive Control | PANC-1, H22, SW1990, LOVO, human liver tissue, human colon cancer tissue, human kidney tissue, mouse kidney tissue, Hela. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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