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| Product Name | Phospho-Erk1(T202)+Erk2(T185) [SZ2-4] |
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| Antibody Type | Primary Antibodies |
| Product description | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr185 of human Erk2. |
| Modification | p-T185 |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 42/44 kDa |
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Application Fig1: ICC staining Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig2: ICC staining Phospho-Erk1(T202)+Erk2(T185) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining Phospho-Erk1(T202)+Erk2(T185) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Erk1(T202)+Erk2(T185) antibody. Counter stained with hematoxylin.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Erk1(T202)+Erk2(T185) antibody. Counter stained with hematoxylin.
Application Fig6: Flow cytometric analysis of MCF-7 cells with Phospho-Erk1(T202)+Erk2(T185) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
| Positive Control | A549, NIH/3T3, MCF-7, human lung cancer tissue, human kidney tissue. |
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| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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