| 存储条件 |
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| Product Name | ATPB [JM10-90] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP 5B antibody ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody ATP synthase subunit beta mitochondrial antibody ATP synthase subunit beta, mitochondrial antibody atp5b antibody ATPB antibody ATPB_HUMAN antibody ATPMB antibody ATPSB antibody Epididymis secretory protein Li 271 antibody HEL-S-271 antibody Mitochondrial ATP synthase beta subunit antibody Mitochondrial ATP Synthase Subunit Beta antibody Mitochondrial ATP synthetase beta subunit antibody |
| Product description | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPICC/IFIHC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:10-1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATP 5B antibody ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody ATP synthase subunit beta mitochondrial antibody ATP synthase subunit beta mitochondrial antibody atp5b antibody ATPB antibody ATPB_HUMAN antibody ATPMB antibody ATPSB antibody Epididymis secretory protein Li 271 antibody HEL-S-271 antibody Mitochondrial ATP synthase beta subunit antibody Mitochondrial ATP Synthase Subunit Beta antibody Mitochondrial ATP synthetase beta subunit antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. |
Application
Fig1: Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T
Application
Fig2: ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Application
Fig11: Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.| Positive Control | 293T, HepG2, A431, Hela, human liver tissue, human kidney tissue, mouse uterus tissue, mouse liver muscle tissue, mouse brain tissue, mouse heart tissue, zebrafish tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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