| 存储条件 |
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| Product Name | M6PR [SR45-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 300 kDa mannose 6 phosphate receptor antibody 300 kDa mannose 6-phosphate receptor antibody Cation independent mannose 6 phosphate receptor antibody Cation-independent mannose-6-phosphate receptor antibody CD222 antibody CD222 antigen antibody CI Man 6 P receptor antibody CI Man-6-P receptor antibody CI MPR antibody CI-M6PR antibody CI-MPR antibody CIMPR antibody IGF 2 receptor antibody IGF 2R antibody IGF II receptor antibody IGF-II receptor antibody IGF2 receptor antibody Igf2r antibody Insulin like growth factor 2 receptor antibody Insulin like growth factor II receptor antibody Insulin-like growth factor 2 receptor antibody Insulin-like growth factor II receptor antibody M6P R antibody M6P/IGF2 receptor antibody M6P/IGF2R antibody M6PR antibody mannose 6 phosphate receptor antibody mannose 6 phosphate receptor, cation independent antibody MPR 300 antibody MPR300 antibody MPRI antibody MPRI_HUMAN antibody |
| Product description | The mannose 6-phosphate/insulin-like growth factor II receptor, IGF-IIR (also designated M6P/IGF2R), is a ubiquitously expressed integral glycoprotein. By binding glycoproteins through two of its extracytoplasmic domains, IGF-IIR mediates the activation of TGF∫1 (a growth inhibitor), the degradation of IGF-II and the transport of lysosomal enzymes. Subsequently, IGF-IIR can form oligomeric complexes, which increase the affinity of IGF-IIR for lysosomal enzymes. Unlike IGF-IR, IGF-IIR does not potentiate the signaling of IGF-I or IGF-II, which have mitogenic, cell survival and insulin-like effects. Therefore, IGF-IIR is characterized as a tumor suppressor. Furthermore, the IGF-IIR gene is located on chromosome 6q26, which is commonly mutated or deleted in several human cancers. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 300 kDa mannose 6 phosphate receptor antibody 300 kDa mannose 6-phosphate receptor antibody Cation independent mannose 6 phosphate receptor antibody Cation-independent mannose-6-phosphate receptor antibody CD222 antibody CD222 antigen antibody CI Man 6 P receptor antibody CI Man-6-P receptor antibody CI MPR antibody CI-M6PR antibody CI-MPR antibody CIMPR antibody IGF 2 receptor antibody IGF 2R antibody IGF II receptor antibody IGF-II receptor antibody IGF2 receptor antibody Igf2r antibody Insulin like growth factor 2 receptor antibody Insulin like growth factor II receptor antibody Insulin-like growth factor 2 receptor antibody Insulin-like growth factor II receptor antibody M6P R antibody M6P/IGF2 receptor antibody M6P/IGF2R antibody M6PR antibody mannose 6 phosphate receptor antibody mannose 6 phosphate receptor cation independent antibody MPR 300 antibody MPR300 antibody MPRI antibody MPRI_HUMAN antibody |
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| Molecular Weight(MW) | 274 kDa |
| Cellular Localization | Lysosome membrane |
Application
Fig1: Western blot analysis of M6PR on different cell lysates using anti-M6PR antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: SW480
Application
Fig2: ICC staining M6PR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining M6PR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining M6PR in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-M6PR antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with M6PR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SW480, MCF-7, Hela, HepG2, human tonsil tissue |
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| Application Notes | WB:1:1,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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