ECK4026 antibody JW3994 antibody Mal E antibody MalE antibody malJ antibody Maltodextrin binding protein antibody Maltose ABC transporter periplasmic protein antibody Maltose binding periplasmic protein antibody MMBP antibody Periplasmic maltose binding protein antibody
Product description
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
Immunogen
recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Recombinant rabbit
Tested Applications
WB
WB:1:1,000-1:5,000
Species Reactivity
Escherichia Coli
Concentration
1mg/ml
Target Information
Alternative Names
ECK4026 antibody JW3994 antibody Mal E antibody MalE antibody malJ antibody Maltodextrin binding protein antibody Maltose ABC transporter periplasmic protein antibody Maltose binding periplasmic protein antibody MMBP antibody Periplasmic maltose binding protein antibody
Fig1: Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.
Positive Control
Recombinant MBP-tag protein.
Application Notes
WB:1:1,000-1:5,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
