Renilla luciferin 2 monooxygenase antibody Renilla type luciferase antibody
Product description
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Recombinant rabbit
Tested Applications
WBICCIF
WB:1:500-1:2,000
Species Reactivity
Sea Anemone
Concentration
1mg/ml
Target Information
Alternative Names
Renilla luciferin 2 monooxygenase antibody Renilla type luciferase antibody
