Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #54470. Specificity / Sensitivity
p38 MAPK (D13E1) Rabbit Monoclonal Antibody recognizes endogenous levels of total p38α, β, or γ MAPK protein. This antibody does not recognize p38δ, SAPK/JNK, or p44/42 MAPK proteins.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Bovine, Pig
Species predicted to react based on 100% sequence homology:
Chicken