Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.
Product Usage Information
Recommended Antibody Dilutions:
1:1000–1:3000
20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K
SignalFire™ ECL Reagent #6883 1:1K–1:3K
SignalFire™ Plus ECL Reagent #12630 1:5K-1:15K
SignalFire™ Elite ECL Reagent #12757 1:10K-1:20K
Storage
Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.
