Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-FoxO1 (Ser256) (E1F7T) Rabbit Monoclonal Antibody recognizes endogenous levels of FoxO1 protein only when phosphorylated at Ser256. This antibody cross-reacts with overexpressed FoxO4 phosphorylated at Ser193 and may cross-react with overexpressed FoxO3a phosphorylated at Ser253. This antibody also cross-reacts with a protein of unknown origin around 160kD.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser256 of human FoxO1 protein.
