Goat Anti-Rabbit IgG (H+L) Antibody is conjugated to Alexa Fluor® 532 fluorescent dye under optimal conditions and formulated at 2 mg/mL.
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for flow cytometry assays.
Storage
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, and 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
Goat Anti-Rabbit IgG (H+L) Antibody (Alexa Fluor® 532 Conjugate) is prepared from goat antibodies and detects the heavy and light chain of rabbit IgG. This antibody conjugate is cross-adsorbed against human IgG, human serum, mouse IgG, mouse serum, and bovine serum.
Species Reactivity:
Rabbit
Background
This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
