Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-c-Myc (Thr244) (F3G5K) Rabbit Monoclonal Antibody recognizes endogenous levels of c-Myc protein only when phosphorylated at Thr244. Thr244 is equivalent to Thr259 in canonical isoform 2 of c-Myc protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr244 of human c-Myc protein.
