For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunofluorescence (Immunocytochemistry)
1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized)
1:50 - 1:200
Chromatin IP
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #46186.
Specificity / Sensitivity
Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of Stat6 protein only when phosphorylated at Tyr641. Weak cross-reactivity with other tyrosine-phosphorylated proteins has been observed in some cell lines.
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology:
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human Stat6 protein.
