For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #CUT&RUN Assay Kit #86652.
Application
Dilution
Western Blotting
1:2000
Simple Western™
1:50 - 1:250
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:200 - 1:800
Immunofluorescence (Immunocytochemistry)
1:600 - 1:1200
Flow Cytometry (Fixed/Permeabilized)
1:400 - 1:800
Chromatin IP
1:100
Chromatin IP-seq
1:100
CUT&RUN
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #23791.
Specificity / Sensitivity
Androgen Receptor (D6F11) Rabbit Monoclonal Antibody detects endogenous levels of total androgen receptor protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.
