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c-Fos (9F6) Rabbit mAb

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
100 µl 现货2-3天 原装正品
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基础信息
存储条件 -20℃
产品详情

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunofluorescence (Frozen)1:1600 - 1:3200
Immunofluorescence (Immunocytochemistry)1:3200 - 1:12800
Flow Cytometry (Fixed/Permeabilized)1:1600 - 1:6400
Chromatin IP1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #
53345.

Specificity / Sensitivity

c-Fos (9F6) Rabbit Monoclonal Antibody detects endogenous levels of total c-Fos protein. This antibody does not cross-react with other Fos proteins, including FosB, FRA1 and FRA2. This antibody non-specifically stains fixed frozen mouse spleen and liver by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat

Species predicted to react based on 100% sequence homology: 

Hamster, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human c-Fos protein.


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