For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Fluorescent Western
1:1000
Immunoprecipitation
1:100
Immunofluorescence (Immunocytochemistry)
1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)
1:400 - 1:1600
Chromatin IP
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #94610.
Specificity / Sensitivity
Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser465/467 of human Smad2 protein.
