Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Akt (E17K Mutant Specific) (D1T7P) Rabbit Monoclonal Antibody recognizes levels of transfected E17K mutant Akt1 and Akt2 protein. This antibody weakly recognizes transfected E17K mutant Akt3.
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E17K mutant sequence of human Akt1 protein.
