Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-Doublecortin (Ser334) (D11B10) Rabbit Monoclonal Antibody recognizes endogenous levels of doublecortin protein only when phosphorylated at Ser334. Amino acid Ser334 of mouse doublecortin corresponds to Ser415 of human doublecortin. Proteins of unknown origin may be detected around 90 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of mouse doublecortin protein.
