This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells and immunofluorescence analysis in human cells and rat tissue. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198.
Product Usage Information
Application
Dilution
Immunofluorescence (Frozen)
1:50 - 1:200
Immunofluorescence (Immunocytochemistry)
1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)
1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of CREB only when phosphorylated at Ser133. This antibody conjugate also recognizes the phosphorylated form of the CREB-related protein, ATF-1.
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Zebrafish
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB protein.
