For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:50
Chromatin IP
1:50
Chromatin IP-seq
1:50
CUT&RUN
1:50
CUT&Tag
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #31624.
Specificity / Sensitivity
Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit Monoclonal Antibody recognizes endogenous levels of Rpb1 only when the carboxy-terminal domain (CTD) heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at Ser2. This antibody does not cross-react with Rpb1 CTD phosphorylated at Ser5 or Ser7.
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:
Hamster, D. melanogaster, Xenopus, Zebrafish, Bovine, Pig, S. cerevisiae, C. elegans
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2 of the human Rpb1 CTD heptapeptide repeat.
