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Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
100 µl 现货2-3天 原装正品
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基础信息
存储条件 -20℃
产品详情

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.


The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.


The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunoprecipitation1:50
Chromatin IP1:50
Chromatin IP-seq1:50
CUT&RUN1:50
CUT&Tag1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #
31624.

Specificity / Sensitivity

Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit Monoclonal Antibody recognizes endogenous levels of Rpb1 only when the carboxy-terminal domain (CTD) heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at Ser2. This antibody does not cross-react with Rpb1 CTD phosphorylated at Ser5 or Ser7.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology: 

Hamster, D. melanogaster, Xenopus, Zebrafish, Bovine, Pig, S. cerevisiae, C. elegans

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2 of the human Rpb1 CTD heptapeptide repeat.


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