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HSF1 (D3L8I) Rabbit mAb

产品编号 : 12972T

英文名称 :

中文名称 : 抗体

品牌 : CST

包装规格	交货周期	质量标准	目录价	会员专享价	数量
20 µl	现货2-3天	原装正品	￥1370	登录后可见	- +

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基础信息产品详情相关文献

基础信息

存储条件	-20℃

产品详情

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:250 - 1:1000
Immunofluorescence (Immunocytochemistry)	1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400
Chromatin IP	1:50
Chromatin IP-seq	1:50
CUT&RUN	1:50
CUT&Tag	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #76196.

Specificity / Sensitivity

HSF1 (D3L8I) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSF1 protein. This antibody is not predicted to cross-react with HSF2.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine, Dog, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HSF1 protein.

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