Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-Vimentin (Ser83) (D5A2D) Rabbit Monoclonal Antibody recognizes endogenous levels of Vimentin protein only when phosphorylated at Ser83.
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human vimentin protein.
