For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:100
Immunofluorescence (Frozen)
1:1600 - 1:3200
Chromatin IP
1:50
Chromatin IP-seq
1:50
CUT&RUN
1:50
CUT&Tag
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #35173.
Specificity / Sensitivity
Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Brn2/POU3F2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro97 of human Brn2/POU3F2 protein.
