For optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunofluorescence (Immunocytochemistry)
1:100 - 1:400
Chromatin IP
1:200
Chromatin IP-seq
1:200
CUT&RUN
1:200
CUT&Tag
1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #82093.
Specificity / Sensitivity
ATF-4 (D4B8) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-4 protein. Reactivity by immunofluorescence is human only.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.
